蒲黄、松花粉等花粉类药材及其混伪品的DNA条形码鉴定(4)
[27]Wang W Q，Wu Y R，Yan Y F，et al. DNA barcoding of the Lemnaceae，a family of Aquatic monocots[J]. BMC Plant Biol，2010，10(1)： 205.
[28]Song J Y，Shi L C，Li D Z，et al. Extensive pyrosequencing reveals frequent intra-genomic variations of internal transcribed spacer regions of nuclear ribosomal DNA[J]. PLoS ONE，2012，7(8)： e43971.
Identification of cattail pollen (Puhuang)，pine pollen (Songhuafen) and
its adulterants by ITS2 sequence
MA Xiao-xi1，2，SUN Wei2，REN Wei-chao2，XIANG Li2，ZHAO Bo2，ZHANG Ya-qin1，SONG Ming1，MU Ze-jing3，CHEN Shi-lin2*
(1. School of Chemical Engineering，Wuhan University of Technology，Wuhan 430070，China；
2.Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China；
3.Jiangxi University of Traditional Chinese Medicine，Nanchang 330004，China)
[Abstract] DNA barcoding method was conducted for the authentication of pollen materials due to difficulty of discriminating pollen materials bearing morphological similarity. In this study，a specific focus was to identify cattail pollen (Puhuang) and pine pollen (Songhuafen) samples from their adulterants which are frequently mixed-together. Regions of the internal transcribed spacer (ITS2) from 60 samples were sequenced，and new primers for cattail pollen were designed according to the sequence information. The results from the NJ trees showed that the species of pine pollen，Puhuang and their adulterants can be classified as obvious monophyly. Therefore，we propose to adapt DNA barcoding methodology to accurately distinguish cattail pollen，pine pollen and their adulterant materials. It is a great help for drug regulatory agency to supervise the quality of medicinal materials.
[Key words] ITS2；cattail pollen；pine pollen；DNA barcode
doi：10.4268/cjcmm20141208, 百拇医药(马孝熙 孙伟 任伟超 向丽 赵博 张雅琴 宋明 慕泽泾 陈士林)
[28]Song J Y，Shi L C，Li D Z，et al. Extensive pyrosequencing reveals frequent intra-genomic variations of internal transcribed spacer regions of nuclear ribosomal DNA[J]. PLoS ONE，2012，7(8)： e43971.
Identification of cattail pollen (Puhuang)，pine pollen (Songhuafen) and
its adulterants by ITS2 sequence
MA Xiao-xi1，2，SUN Wei2，REN Wei-chao2，XIANG Li2，ZHAO Bo2，ZHANG Ya-qin1，SONG Ming1，MU Ze-jing3，CHEN Shi-lin2*
(1. School of Chemical Engineering，Wuhan University of Technology，Wuhan 430070，China；
2.Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China；
3.Jiangxi University of Traditional Chinese Medicine，Nanchang 330004，China)
[Abstract] DNA barcoding method was conducted for the authentication of pollen materials due to difficulty of discriminating pollen materials bearing morphological similarity. In this study，a specific focus was to identify cattail pollen (Puhuang) and pine pollen (Songhuafen) samples from their adulterants which are frequently mixed-together. Regions of the internal transcribed spacer (ITS2) from 60 samples were sequenced，and new primers for cattail pollen were designed according to the sequence information. The results from the NJ trees showed that the species of pine pollen，Puhuang and their adulterants can be classified as obvious monophyly. Therefore，we propose to adapt DNA barcoding methodology to accurately distinguish cattail pollen，pine pollen and their adulterant materials. It is a great help for drug regulatory agency to supervise the quality of medicinal materials.
[Key words] ITS2；cattail pollen；pine pollen；DNA barcode
doi：10.4268/cjcmm20141208, 百拇医药(马孝熙 孙伟 任伟超 向丽 赵博 张雅琴 宋明 慕泽泾 陈士林)